Addressing Challenges in Ion-Selectivity Characterization in Nanopores.

Ion selectivity is the basis for designing smart nanopore/channel-based devices, e.g., ion separators and biosensors. Quantitative characterization of ion selectivities in nanopores often employs the Nernst or Goldman-Hodgkin-Katz (GHK) equation to interpret transmembrane potentials. However, the direction of the measured transmembrane potential drop is not specified in these equations, and selectivity values calculated using absolute values of transmembrane potentials do not directly reveal the ion for which the membrane is selective. Moreover, researchers arbitrarily choose whether to use the Nernst or GHK equation and overlook the significant differences between them, leading to ineffective quantitative comparisons between studies. This work addresses these challenges through (a) specifying the transmembrane potential (sign) and salt concentrations in terms of working and reference electrodes and the solutions in which they reside when using the Nernst and GHK equations, (b) reporting of both Nernst-selectivity and GHK-selectivity along with solution compositions and transmembrane potentials when comparing different nanopores/channels, and (c) performing simulations to define an ideal selectivity for nanochannels. Experimental and modeling studies provide significant insight into these fundamental equations and guidelines for the development of nanopore/channel-based devices.

Environmental-friendly, flexible silk fibroin-based film as dual-responsive shape memory material.

Bio-based shape memory materials have attracted wide attention due to their biocompatibility, degradability and safety. However, designing and manufacturing wearable bio-based shape memory films with excellent flexibility and toughness is still a challenge. In this work, silk fibroin substrate with a ß-sheet structure was combined with a tri-block shape memory copolymer to prepare a transparent composited shape memory film. The silk fibroin-based film showed a dual-responsive shape memory function, which can respond to both temperature and water stimuli. This film has a sensitive water-responsive shape memory, which starts deforming after exposure to water for 3 s and fully recovers in 30 s. In addition, the composite film shows highly stretchable (>300 %) and could maintain its high tensile properties after 5 cycles of regeneration. The films also exhibited rapid degradation ability. This study provides new insights for the design of dual-responsive shape memory materials by combining biocompatible matrix and multi-block SMP to simultaneously enhance the mechanical properties, which can be used for intelligent packaging, medical supplies, soft actuators and wearable devices.

Pore-Size-Dependent Role of Functional Elements at the Outer Surface and Inner Wall in Single-Nanochannel Biosensors.

Biological nanopores feature functional elements on the outer surfaces (FEOS) and inner walls (FEIW), enabling precise control over ions and molecules with exceptional sensitivity and specificity. This provides valuable inspiration to scientists for the development of intelligent artificial nanochannel-based platforms, with a wide range of potential applications, including biosensors. Much effort has been dedicated to investigating the distinct contribution of FEOS and FEIW of multichannel membrane biosensors. However, the intricate interactions among neighboring pores in multichannel biosensors have presented challenges. This underscores the untapped potential of single nanochannels as ideal candidates in this field. Here, we employed single nanochannel membranes with different pore sizes to investigate the distinct contributions of FEIW and FEOS to single-nanochannel biosensors, combined with numerical simulations. Our findings revealed that alterations in the negative charges of FEIW and FEOS, induced by target binding, have differential effects on ion transport, contingent upon the degree of nanoconfinement. In the case of smaller pores, such as 20 nm, the ion concentration polarization driven by FEIW can independently control ion transport through the surface's electric double layer. However, as the pore size increases to 40-60 nm, both FEIW and FEOS become essential for effective ion concentration polarization. When the pore size reaches 100 nm, both FEIW and FEOS are ineffective and thus unsuitable for biosensors. Simulations demonstrate that the observed phenomena can be attributed to the interactions between the charges of FEIW and FEOS within the overlapping electric double layer under confinement. These results underscore the critical role of pore size as a key parameter in governing the functionality of probes within or on nanopore-based biosensors as well as in the design of nanopore-based devices.

Effect of web-implemented exercise interventions on depression and anxiety in patients with neurological disorders: a systematic review and meta-analysis.

Introduction: Web-implemented exercise intervention is the latest and innovative method to improve people's mental health. Currently, many studies have proven that web-implemented interventions are effective to improve depression and anxiety in adults. However, the influence of different web-implemented exercise interventions on depression and anxiety in patients with neurological disorders is still unclear. Objective: The study aims to systematically summarize the type and content of web-implemented exercise interventions and quantify the effect of different web-implemented exercise interventions on depression and anxiety in patients with neurological disorders. Methods: Four literature databases (PubMed, Web of Science, China National Knowledge Infrastructure, and WanFang data) were searched. The literature search considered studies published in English or Chinese before October 13, 2022. Randomized controlled trials (RCTs) that participants accepted web-implemented interventions were included. Two authors independently extracted data and assessed the risk of bias for included studies. Standardized mean differences (SMD) with 95% CI were used to integrate the effect size. Results: 16 RCTs (a total of 963 participants) were included. The results showed that web-implemented exercise intervention had a significant effect on depression (SMD = -0.80; 95% CI, -1.09 to -0.52; I2 = 75%; P < 0.00001) and anxiety (SMD = -0.80; 95% CI, -1.23 to -0.36; I2 = 75%; P = 0.0003) in patients with a neurological disorder. The subgroup analysis showed that the effectiveness of the web-implemented exercise intervention was influenced by several factors, such as web-implemented exercise intervention type, component, and intervention duration. Conclusion: Web-implemented exercise intervention has a relieving effect on depression and anxiety symptoms in patients with neurological disorders. Additionally, the intervention type, intervention duration, and component can influence the effect size. Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier: CRD42023409538.

How to Test the On-Ice Aerobic Capacity of Speed Skaters? An On-Ice Incremental Skating Test for Young Skaters.

Aerobic capacity is important for speed skaters to achieve good results in middle-long distance events. The technical characteristics of speed skating cause intermittent blood flow blockage in the lower limbs. Therefore, an athlete's aerobic capacity on ice may differ from that measured by cycling or running. Now, the on-ice aerobic capacity lacks methods for conducting aerobic capacity tests on ice. Objective: The objective of this study was to develop a method for measuring on-ice aerobic capacity for young athletes and to compare it with the VO2max test on cycling. Methods: This study established a test method for the on-ice aerobic capacity of young, high-level speed skaters with incremental load (on-ice incremental skating test, OIST) through expert interviews and literature review. In the first part, OIST was used to test the aerobic abilities of 65 youth professional speed skaters (51 males and 14 females) on ice and to explore the correlation with their specific performance. The second part compares the relationship between aerobic capacity on ice and aerobic capacity on bicycle of 18 young high-level male athletes. The third part establishes the regression formula of ice ventilation threshold heart rate. The OIST established in this study can evaluate the on-ice aerobic capacity of athletes from National Level and Level 1&2 in China. The athletes' on-ice aerobic capacity indicators were significantly lower than those of the cycling test. However, the values of absolute VO2max and absolute ventilatory threshold had a high correlation (R = 0.532, p < 0.05; R = 0.584, p < 0.05). The regression formula of ventilatory threshold heart rate on ice = 0.921 × HRmax (Cycling test) -9.243. The OIST established in this study meets the characteristics and requirements of the VO2max measurement method. The OIST seems to be able to better evaluate the aerobic capacity of athletes skating on ice. The indicators of maximum oxygen uptake and ventilation threshold in OIST were significantly lower than those in the aerobic cycling test, but there was a good correlation. The aerobic cycling test can be used as an important selection index of the ice aerobic capacity of speed skaters. The regression formula will provide an important basis for coaches to accurately monitor the intensity of ice training.

Synthetic K+ Channels Constructed by Rebuilding the Core Modules of Natural K+ Channels in an Artificial System.

Different types of natural K+ channels share similar core modules and cation permeability characteristics. In this study, we have developed novel artificial K+ channels by rebuilding the core modules of natural K+ channels in artificial systems. All the channels displayed high selectivity for K+ over Na+ and exhibited a selectivity sequence of K+ ≈Rb+ during the transport process, which is highly consistent with the cation permeability characteristics of natural K+ channels. More importantly, these artificial channels could be efficiently inserted into cell membranes and mediate the transmembrane transport of K+ , disrupting the cellular K+ homeostasis and eventually triggering the apoptosis of cells. These findings demonstrate that, by rebuilding the core modules of natural K+ channels in artificial systems, the structures, transport behaviors, and physiological functions of natural K+ channels can be mimicked in synthetic channels.

Correction: Polyaniline nanorods dotted on graphene oxide nanosheets as a novel super adsorbent for Cr(VI).

Correction for 'Polyaniline nanorods dotted on graphene oxide nanosheets as a novel super adsorbent for Cr(VI)' by Shouwei Zhang et al., Dalton Trans., 2013, 42, 7854-7858, https://doi.org/10.1039/C3DT50149C.

Influence of handrail height and knee joint support on sit-to-stand movement.

Handrail height and knee joint support both significantly influence sit-to-stand (STS) movement. However, research on the associations between handrail height, knee joint support, and their cumulative effect on STS kinematics and changes in plantar pressure distribution during STS under different handrail heights and knee joint support is still unclear. The main objective of this study was to examine the influence of handrail height and knee joint support on the kinematics and the distribution of plantar pressure in healthy adults during STS. Twenty-six healthy adult subjects aged 23 to 58 years participated in this experiment. The subjects carried out STS movement experiments under 7 conditions: 6 experimental conditions of 3 different heights of handrail, with and without knee joint support, and 1 control condition of standing up naturally. The motions of the markers were recorded using cameras operating at 60 Hz, and total movement time, the percentage of movement time of each phase, trunk tilt angle, joint angle, plantar pressure, and the time from hindfoot to forefoot peak pressure were analyzed and compared. Handrail height significantly influences the percentage of movement time at phase I (P = .015) and the maximum trunk tilt angle (P < .05), knee joint support significantly influences the maximum trunk tilt angle and ankle angle (P = .033), and handrail height and knee joint support have an interaction on the time from hindfoot to forefoot peak pressure (P < .001). Subjects' STS performance was improved with the use of assistant devices but showed particular improvement under the condition of with knee joint support when the handrail height was middle handrail.

A Flexible TENG Based on Micro-Structure Film for Speed Skating Techniques Monitoring and Biomechanical Energy Harvesting.

Wearable motion-monitoring systems have been widely used in recent years. However, the battery energy storage problem of traditional wearable devices limits the development of human sports training applications. In this paper, a self-powered and portable micro-structure triboelectric nanogenerator (MS-TENG) has been made. It consists of micro-structure polydimethylsiloxane (PDMS) film, fluorinated ethylene propylene (FEP) film, and lithium chloride polyacrylamide (LiCl-PAAM) hydrogel. Through the micro-structure, the voltage of the MS-TENG can be improved by 7 times. The MS-TENG provides outstanding sensing properties: maximum output voltage of 74 V, angular sensitivity of 1.016 V/degree, high signal-to-noise ratio, and excellent long-term service stability. We used it to monitor the running skills of speed skaters. It can also store the biomechanical energy which is generated in the process of speed skating through capacitors. It demonstrates capability of sensor to power electronic calculator and electronic watch. In addition, as a flexible electrode hydrogel, it can readily stretch over 1300%, which can help improve the service life and work stability of MS-TENG. Therefore, MS-TENG has great application potential in human sports training monitoring and big data analysis.

CONSTANS Polymorphism Modulates Flowering Time and Maturity in Soybean.

CONSTANS (CO) plays a critical role in the photoperiodic flowering pathway. However, the function of soybean CO orthologs and the molecular mechanisms in regulating flowering remain largely unknown. This study characterized the natural variations in CO family genes and their association with flowering time and maturity in soybeans. A total of 21 soybean CO family genes (GmCOLs) were cloned and sequenced in 128 varieties covering 14 known maturity groups (MG 0000-MG X from earliest to latest maturity). Regarding the whole genomic region involving these genes, GmCOL1, GmCOL3, GmCOL8, GmCOL9, GmCOL10, and GmCOL13 were conserved, and the remaining 15 genes showed genetic variation that was brought about by mutation, namely, all single-nucleotide polymorphisms (SNPs) and insertions-deletions (InDels). In addition, a few genes showed some strong linkage disequilibrium. Point mutations were found in 15 GmCOL genes, which can lead to changes in the potential protein structure. Early flowering and maturation were related to eight genes (GmCOL1/3/4/8/13/15/16/19). For flowering and maturation, 11 genes (GmCOL2/5/6/14/20/22/23/24/25/26/28) expressed divergent physiognomy. Haplotype analysis indicated that the haplotypes of GmCOL5-Hap2, GmCOL13-Hap2/3, and GmCOL28-Hap2 were associated with flowering dates and soybean maturity. This study helps address the role of GmCOL family genes in adapting to diverse environments, particularly when it is necessary to regulate soybean flowering dates and maturity.

Mutational landscape of circulating tumor DNA identifies distinct molecular features associated with therapeutic response in patients with metastatic colorectal cancer.

BACKGROUND: We investigated the mutational landscape of circulating tumor DNA (ctDNA) in predicting tumor response to first-line treatment in patients with metastatic colorectal cancer (mCRC). METHODS: We included 41 patients with initially unresectable mCRC, treated with 5-fluorouracil/leucovorin/oxaliplatin (FOLFOX)/5-fluorouracil/leucovorin/irinotecan (FOLFIRI) with/without bevacizumab (Bev)/cetuximab (Cet). Blood samples were prospectively collected at two timepoints: at baseline and after four cycles of first-line treatment. Mutational status of 1086 genes were studied in ctDNA by targeted next-generation sequencing (NGS). Molecular mutational burden (MMB) was defined as mean mutation frequency among obtained mutations for each gene. To evaluate the association between molecular characteristics of cfDNA and therapeutic response better, we divided these patients into MMB-high and MMB-low group according to the median value of MMB (0.3). RESULTS: Among the 41 enrolled patients, alterations of six genes (TRIM24, SPEN, RNF43, PRKAR1A, KRAS, and KDM5 C) were found at baseline. Baseline MMB of six genes was significantly lower in partial response (PR)/stable disease (SD) patients than progression disease (PD) patients (p = 0.0012). Further analysis demonstrated that genomic profiling of ctDNA from pretreatment blood samples was significantly different between PR/SD (non-PD) group and PD group. By comparing the baseline levels of KRAS MMB in the two subgroups, we found that PD cases were all MMB-high, whereas non-PD cases were mainly in MMB-low subgroup. Furthermore, patients with low-KRAS MMB had superior response rate, significantly longer progression-free survival (PFS) and longer overall survival (OS) than high-KRAS MMB group. CONCLUSIONS: This prospective and serial genomic profiling study revealed the utility of ctDNA in predicting clinical outcomes in mCRC patients under first-line treatment. Levels of KRAS MMB might aid in monitoring therapeutic efficacy in mCRC patients at pretreatment/after four cycles of first-line treatment.

Hierarchical multi-active component yolk-shell nanoreactors as highly active peroxymonosulfate activator for ciprofloxacin degradation.

The reasonable design of the structure and composition of catalysts was essential to improve the catalytic performance of advanced oxidation processes (AOPs). Herein, we reported a simple strategy to synthesize hierarchical Co3O4-C@CoSiOx yolk-shell nanoreactors with multiple active components by using metal-organic frameworks (MOFs). The novel nanoreactors are further used to activate peroxymonosulfate (PMS) for ciprofloxacin (CIP) degradation. The effects of reaction parameters (pH value, co-existing ions, reaction temperature, etc.) on CIP degradation were systematically investigated. Especially, ∼98.2% of CIP was degraded within 17 min under the optimal conditions, together with the low cobalt leaching and excellent reusability. The appreciable catalytic performance improvement might be due to the synergistic effect of the structure and component design: (1) the hierarchical yolk-shell structure endowed the catalyst with high surface area (∼232.47 m2/g) and fully exposed active sites; (2) abundant highly active ≡Co-OH+ were formed on the surface of CoSiOx; (3) the presence of oxygen vacancies and nitrogen-doped carbon promoted the decomposition of PMS through a non-radical process. The results revealed both the radical (SO4∙-, ∙OH and O2∙-) and non-radical (1O2 and direct charge transfer) should be responsible for the CIP degradation. Moreover, the possible degradation pathways of CIP were proposed through the identification of intermediates using LC-MS/MS techniques and density functional theory (DFT) calculation. Our work highlights that multi-component catalysts derived from MOFs with novel structure have broad application prospects in AOPs.

Effect of different handrail types and seat heights on kinematics and plantar pressure during STS in healthy young adults.

ABSTRACT: Handrail type and seat height both significantly influence sit-to-stand (STS) movement. However, research on the associations between handrail type, seat height, and their cumulative effect on STS kinematics and changes in plantar pressure distribution during STS under different handrail types and seat heights is insufficient.The main objective of this study was to investigate the effect of different handrail types and seat heights on the kinematics and plantar pressure in healthy adults during STS.The study was conducted on 26 healthy young adults. Six conditions were tested: low seat (LS) and vertical handrail; LS and horizontal handrail (HH); LS and bilateral handrail; high seat (HS) and vertical handrail; HSHH; HS and bilateral handrail. The movement time, trunk tilt angle, and time from hindfoot to forefoot peak pressure were analyzed and compared.A significant difference was found in handrail type (P < .001) and seat height (P < .02) for the total movement time of STS. A significant difference was also found for the maximum trunk tilt angles (P < .001) in handrail types. There was an interaction between handrail type and seat height for the time from hindfoot to forefoot peak pressure of STS (P = .003).Using HSHH could take less time to accomplish STS movement; it also reduced the maximum trunk tilt angle and thus reduce the risk of falling; the time from hindfoot to forefoot peak pressure when using HSHH was short and subjects could accomplish STS movement easier.

Retentive capacity of power output and linear versus non-linear mapping of power loss in the isotonic muscular endurance test.

The limit of dynamic endurance during repetitive contractions has been referred to as the point of muscle fatigue, which can be measured by mechanical and electrophysiological parameters combined with subjective estimates of load tolerance for revealing the human real-world capacity required to work continuously. In this study, an isotonic muscular endurance (IME) testing protocol under a psychophysiological fatigue criterion was developed for measuring the retentive capacity of the power output of lower limb muscles. Additionally, to guide the development of electrophysiological evaluation methods, linear and non-linear techniques for creating surface electromyography (sEMG) models were compared in terms of their ability to estimate muscle fatigue. Forty healthy college-aged males performed three trials of an isometric peak torque test and one trial of an IME test for the plantar flexors and knee and hip extensors. Meanwhile, sEMG activity was recorded from the medial gastrocnemius, lateral gastrocnemius, vastus medialis, rectus femoris, vastus lateralis, gluteus maximus, and biceps femoris of the right leg muscles. Linear techniques (amplitude-based parameters, spectral parameters, and instantaneous frequency parameters) and non-linear techniques (a multi-layer perception neural network) were used to predict the time-dependent power output during dynamic contractions. Two mechanical manifestations of muscle fatigue were observed in the IME tests, including power output reduction between the beginning and end of the test and time-dependent progressive power loss. Compared with linear mapping (linear regression) alone or a combination of sEMG variables, non-linear mapping of power loss during dynamic contractions showed significantly higher signal-to-noise ratios and correlation coefficients between the actual and estimated power output. Muscular endurance required in real-world activities can be measured by considering the amount of work produced or the activity duration via the recommended IME testing protocol under a psychophysiological termination criterion. Non-linear mapping techniques provide more powerful mapping of power loss compared with linear mapping in the IME testing protocol.

Modified chemical reaction optimization and its application in engineering problems.

Chemical Reaction Optimization (CRO) is a simple and efficient evolutionary optimization algorithm by simulating chemical reactions. As far as the current research is concerned, the algorithm has been successfully used for solving a number of real-world optimization tasks. In our paper, a new real encoded chemical reaction optimization algorithm is proposed to boost the efficiency of the optimization operations in standard chemical reactions optimization algorithm. Inspired by the evolutionary operation of the differential evolution algorithm, an improved search operation mechanism is proposed based on the underlying operation. It is modeled to further explore the search space of the algorithm under the best individuals. Afterwards, to control the perturbation frequency of the search strategy, the modification rate is increased to balance between the exploration ability and mining ability of the algorithm. Meanwhile, we also propose a new population initialization method that incorporates several models to produce high-quality initialized populations. To validate the effectiveness of the algorithm, nine unconstrained optimization algorithms are used as benchmark functions. As observed from the experimental results, it is evident that the proposed algorithm is significantly better than the standard chemical reaction algorithm and other evolutionary optimization algorithms. Then, we also apply the proposed model to address the synthesis problem of two antenna array synthesis. The results also reveal that the proposed algorithm is superior to other approaches from different perspectives.

Retraction: Polymer nanodots of graphitic carbon nitride as effective fluorescent probes for the detection of Fe3+ and Cu2+ ions.

Retraction of 'Polymer nanodots of graphitic carbon nitride as effective fluorescent probes for the detection of Fe3+ and Cu2+ ions' by Shouwei Zhang et al., Nanoscale, 2014, 6, 4157-4162, DOI: 10.1039/C3NR06744K.

NEAT1 silencing alleviates pulmonary arterial smooth muscle cell migration and proliferation under hypoxia through regulation of miR­34a­5p/KLF4 in vitro.

Pulmonary arterial hypertension (PAH) is a severe vascular disease that adversely affects patient health and can be life threatening. The present study aimed to investigate the detailed role of nuclear paraspeckle assembly transcript 1 (NEAT1) in PAH. Using RT­qPCR, the expression levels of NEAT1, microRNA (miR)­34a­5p, and Krüppel­like factor 4 (KLF4) were detected in both hypoxia­treated pulmonary arterial smooth muscle cells (PASMCs) and serum from PAH patients. Then, the interactions among miR­34a­5p, NEAT1, and KLF4 were evaluated by dual­luciferase reporter assay. The detailed role of the NEAT1/miR­34a­5p/KLF4 axis in PAH pathogenesis was further explored using MTT, Transwell, and western blot assays. The results revealed that NEAT1 targeted miR­34a­5p and miR­34a­5p targeted KLF4. In hypoxia­treated PASMCs and serum from PAH patients, high NEAT1 and KLF4 expression levels and low miR­34a­5p expression were observed. The proliferation and migration of hypoxia­treated PASMCs were reduced by transfection with sh­NEAT1 or miR­34a­5p mimics. The suppressive effects of NEAT1 knockdown on the proliferation and migration of hypoxia­treated PASMCs were reversed by knock down of miR­34a­5p expression and increased KLF4 expression. NEAT1 was not only highly expressed in the serum of PAH patients but its silencing also alleviated PAH by regulating miR­34a­5p/KLF4 in vitro. The present study highlighted a potential new therapeutic target and diagnostic biomarker for PAH.

Kinematic analysis of the human body during sit-to-stand in healthy young adults.

ABSTRACT: Sit-to-stand (STS) motion is one of the most important and energy-consuming basic motions in everyday life. Kinematic analysis provides information regarding what strategy or motion pattern is used by the healthy people, and through which, we can understand and obtain the law of the STS motion. The objective of this article is to study the law of STS motion through the experiment to determine a suitable description of STS motion in healthy adults, so as to provide a starting point and bases for future design and control of STS assistive devices.Thirty healthy adult subjects participated in this study and carried out STS motion experiment of standing up naturally. The STS motions were recorded using a high-definition camera. The experimentally collected kinematic data and a link segment model of the human body were used to obtain the coordinates of joints and to calculate the coordinates, velocity, and momentum of center of gravity; the postures of human body during STS are also obtained. The relationship between human body parameters and motion parameters is analyzed by using Pearson correlation method.The STS motion is divided into 4 phases; the phases are differentiated in terms of STS motion characteristics and postures, and momentum of center of gravity of human body. The main factors determining the differences in STS motion among individuals are horizontal distance between hip joint and ankle joint, lower leg length, thigh length, and the length of the transition period. The horizontal distance between hip joint and ankle joint is positively correlated with the duration from motion begin to trunk stops flexing forward (P = .021 < .05), but not so with the duration from motion begin to the end of phase 2 (P = .15 > .05).The results suggest that when designing the sit-to-stand assistive devices, one should pay attention to the whole-body posture control in STS motion, such as the posture guidance of trunk and lower leg, and should carry out specific training according to different STS phases. Sit-to-stand assistive devices should provide the same horizontal distance between hip joint and ankle joint for different individuals during the STS motion. Transition period should be properly controlled, and the degree of freedom of the lower leg should not be limited.

Layer-by-layer assembly in nanochannels: assembly mechanism and applications.

Layer-by-layer (LbL) assembly is a versatile technology to construct multifunctional nanomaterials using various supporting substrates, enabled by the large selection freedom of building materials and diversity of possible driving forces. The fine regulation over the film thickness and structure provides an elegant way to tune the physical/chemical properties by mild assembly conditions (e.g. pH, ion strength). In this review, we focus on LbL in nanochannels, which exhibit a different growth mechanism compared to "open", convex substrates. The assembly mechanism in nanochannels is discussed in detail, followed by the summary of applications of LbL assemblies liberated from nanochannel templates which can be used as nanoreactors, drug carriers and transporting channels across cell membranes. For fluidic applications, robust membrane substrates are required to keep in place nanotube arrays for membrane-based separation, purification, biosensing and energy harvesting, which are also discussed. The good compatibility of LbL with crossover technologies from other fields allows researchers to further extend this technology to a broader range of research fields, which is expected to result in an increased number of applications of LbL technology in the future.

Highly Rectifying Fluidic Diodes Based on Asymmetric Layer-by-Layer Nanofilms on Nanochannel Membranes.

Nanochannel-based fluidic diodes display ion selectivity and ion current rectification (ICR), which may prove to be important in energy-harvesting devices and biosensors. This paper reports asymmetric functionalization of the outer surface of a flexible nanochannel polymer membrane to create fluidic diodes that give ICR. Layer-by-layer (LbL) adsorption with cross-linking of only the underlying part of the polyelectrolyte nanofilm leads to a porosity step across the film. The combination of a high effective surface charge density and the porosity step in the film leads to a remarkable maximum ICR factor of ∼200 with a pH gradient across the film. Incorporation of pH-sensitive polyelectrolyte components enables the ICR factor to increase an order of magnitude on going from pH 8 to pH 3. Moreover, the coated membrane shows excellent anion selectivity. Thus, LbL adsorption with partial cross-linking provides a simple method for creating coated nanochannel membranes that serve as pH-responsive ionic diodes for potential chemical/biosensors.